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Invidious > Channel > the bumbling biochemist

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YT 39 minutes 58 seconds the bumbling biochemist Recombinant protein expression in bacteria using the inducible T7/pET system YT 43 minutes 4 seconds the bumbling biochemist Baculovirus Expression Vector Systems - expressing proteins in insect cells - theory & practice YT 46 minutes 54 seconds the bumbling biochemist Troubleshooting troublesome recombinant protein expression...

YT 35 minutes 51 seconds the bumbling biochemist Molecular cloning overview - techniques & workflow YT 10 minutes 36 seconds the bumbling biochemist What to do when a plasmid comes for you: agar stab to plate to glycerol stock, miniprep & sequencing YT 44 minutes 37 seconds the bumbling biochemist Choosing vector plasmids and subcloning

YT 24 minutes 2 seconds the bumbling biochemist Bacterial expression strains - esp BL21 strains with plasmids containing tRNAs for rare codons YT 23 minutes 1 second the bumbling biochemist Bacterial growth media - terms, types (LB, TB, SOC, etc.), and usages in biochemistry YT 20 minutes 30 seconds the bumbling biochemist HEK 293 cells: types and uses

YT 23 minutes 15 seconds the bumbling biochemist Expasy ProtParam: pI (isoelectric point), extinction coefficient for UV-based concentration, etc. YT 14 minutes 55 seconds the bumbling biochemist Protein prep inventory-ing: keeping a detailed inventory of cell pellets and purified proteins YT 21 minutes 32 seconds the bumbling biochemist Keeping a detailed plasmid inventory

YT 1 hour 2 minutes 53 seconds the bumbling biochemist Experimental techniques: overview of biochemistry, molecular biology & structural biology techniques YT 32 minutes 15 seconds the bumbling biochemist Codon Optimization & Codon Usage Bias YT 35 minutes 56 seconds the bumbling biochemist Cell-free protein expression & the biochemistry of the PURE expression system

YT 40 minutes 19 seconds the bumbling biochemist Unnatural/non-canonical amino acid incorporation by "genetic code expansion" inspired by pyrrolysine YT 31 minutes 45 seconds the bumbling biochemist Mutant-making: protein expression construct terms and site-directed mutagenesis YT 23 minutes 9 seconds the bumbling biochemist bacterial protein overexpression

YT 29 minutes 17 seconds the bumbling biochemist Troubleshooting protein expression YT 55 minutes 20 seconds the bumbling biochemist Pyrrolysine and noncanonical incorporation with PylRS + PyltRNA & amber suppression YT 31 minutes 36 seconds the bumbling biochemist What do structural biologists do?

YT 26 minutes 28 seconds the bumbling biochemist Fusion partners for improving solubility & expression of recombinant proteins - SUMO, GST, MBP, etc. YT 20 minutes 48 seconds the bumbling biochemist Sequencing your plasmids (emphasis on Sanger sequencing) YT 44 minutes 31 seconds the bumbling biochemist SLIC cloning (Sequence and Ligation Independent Cloning) theory & workflow

YT 29 minutes 20 seconds the bumbling biochemist cDNA (complementary DNA), its uses, & some nuances to take into account when molecular cloning YT 26 minutes 31 seconds the bumbling biochemist Bacterial growth media (LB, TB, SOB, SOC, etc.) YT 49 minutes 54 seconds the bumbling biochemist Inducible protein overexpression in bacteria with the T7 expression system

YT 37 minutes 25 seconds the bumbling biochemist Protein expression & purification techniques - overview of some common techniques & typical workflow YT 7 minutes 37 seconds the bumbling biochemist IPTG-inducible protein expression using the lac promoter: just-the-gist version YT 11 minutes 33 seconds the bumbling biochemist OD (Optical Density) vs absorbance in biochemistry & OD600 monitoring of bacterial growth

YT 8 minutes 20 seconds the bumbling biochemist IPTG induction using the lac promoter YT 10 minutes 6 seconds the bumbling biochemist Lab tip: Check pre-& post-induction samples on an SDS-PAGE gel to see if your protein expressed YT 12 minutes 38 seconds the bumbling biochemist Terrific Broth (TB media) - when/why to use it and how to prepare it

YT 50 minutes 44 seconds the bumbling biochemist Overview of a "typical" recombinant protein expression & purification workflow YT 1 minute 48 seconds the bumbling biochemist Lab tip: stick an incubator in a cold room if you need to go ↓ RT but don’t have refrigerated one YT 44 minutes 30 seconds the bumbling biochemist Overview of recombinant protein expression, purification, & structural biology

YT 6 minutes 39 seconds the bumbling biochemist Autoinduction for recombinant protein overexpression YT 11 minutes 5 seconds the bumbling biochemist Recombinant protein expression and purification overview (quick version)